Flow Injection-Acid Hydrolysis Technique for Determination of Organic Phosphates.
نویسندگان
چکیده
منابع مشابه
Flow injection spectrophotometric determination of acetylsalicylic acid in tablets after on-line microwave-assisted alkaline hydrolysis
The proposed method is based on the on-line microwave-assisted alkaline hydrolysis of acetylsalicylic acid (ASA) to salicylic acid (SA) that reacts with Fe to form a complex that absorbs at 525 nm. Samples merged with NaOH were passed continuously through a domestic microwave oven in order to accelerate the hydrolysis of ASA. Under the best analytical conditions, the linearity of the calibratio...
متن کاملDetermination of Binding Constants by Flow Injection Gradient Technique
A new method is based on the flow injection gradient equipped with an acousto-optic tunable filter (AOTF) detector has been developed for the determination of binding constants of substrates with cyclodextrins or micelles. The concentration gradient produced by this method eliminates the need to prepare series of mixtures in which the concentrations of one of the reactants are varied. The fast ...
متن کاملEffect of organic phosphates on methemoglobin reduction by ascorbic acid.
The rate of methemoglobin reduction by ascorbic acid was accelerated in the presence of ATP,2,3-diphosphoglycerate (2,3-DPG), and inositol hexaphosphate (IHP). The acceleration was as much as three times, four times, and ten times in the presence of ATP, 2.3-DPG, and IHP at pH 7.0, respectively. The changes of the concentrations of methemoglobin and ascorbic acid during the methemoglobin reduct...
متن کاملThermodynamics of hydrolysis of sugar phosphates.
Thermodynamics of the enzyme-catalyzed (alkaline phosphatase, EC 3.1.3.1) hydrolysis of glucose 6-phosphate, mannose 6-phosphate, fructose 6-phosphate, ribose 5-phosphate, and ribulose 5-phosphate have been investigated using microcalorimetry and, for the hydrolysis of fructose 6-phosphate, chemical equilibrium measurements. Results of these measurements for the processes sugar phosphate2- (aqu...
متن کاملAlkaline phosphatase revisited: hydrolysis of alkyl phosphates.
Escherichia coli alkaline phosphatase (AP) is the prototypical two metal ion catalyst with two divalent zinc ions bound approximately 4 A apart in the active site. Studies spanning half a century have elucidated many structural and mechanistic features of this enzyme, rendering it an attractive model for investigating the potent catalytic power of bimetallic centers. Unfortunately, fundamental ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Sciences
سال: 1993
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.9.397